Advanced Genetic Sequencing (Research Support)

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Country: USA
Summary: Advanced Genetic Sequencing (Research Support)
UST Ref No: 124469114
Deadline: 20 Aug 2025
Competition: ICB
Financier: Self Financed
Purchaser Ownership: Public
Tender Value: Refer Document
Notice Type: Tender
Document Ref. No.: 36C24E25Q0045
Purchaser's Detail :

Purchaser : VETERANS AFFAIRS, DEPARTMENT OF
323 NORTH SHORE DRIVE SUITE 500, PITTSBURGH , PA 15212, USA
Primary Point of Contact: Ms. Millicent Covert, millicent.covert@va.gov, Phone Number (Phone calls will not be taken)
Email :millicent.covert@va.gov

Description :
Description
RFQ 36C24E25Q0045Advanced Genetic Sequencing Solicitation Clarification and Question & Answer, PART 3**Quote submission is extended to no later than 11:59AM EST, Wednesday, August 20, 2025.**Quotes shall be submitted via email to millicent.covert@va.gov. Offerors must reference Solicitation 36C24E25Q0045 in the subject line of the email. No telephone requests for information will be considered. Incomplete packages will be considered non-responsive.______________________________________________________________________This project requires high-throughput, high-quality single-cell RNA sequencing (scRNA-seq) services compatible with Parse Biosciences library preparation technology for murine brain tissue samples. The sequencing provider must meet specific technical requirements necessary for data quality, compatibility, and compliance with VA standards. These include the ability to accommodate Parse's unique chemistry, ensure precise read configurations, and deliver data in a timely and secure manner. Our specifications are designed to eliminate ambiguity, ensure an equitable vendor comparison, and reflect scientifically justified parameters aligned with our research goals.Technical Requirements1. Specialized Sequencing CompatibilityParse Biosciences uses a non-standard read configuration:Read 1: 28 bp (cell barcode + UMI)Read 2: 91 100 bp (transcript)Index i7: 8 bp (sample index)The sequencing provider must demonstrate prior experience or validated capability with Parse Biosciences libraries and must support these custom read configurations on Illumina platforms.2. High-Throughput Capacity with Fast TurnaroundFor each sample consisting of 10,000 nuclei sequenced at 75,000 reads per cell,
750 million paired-end reads are required.The provider must offer access to NovaSeq S1 or S2 lanes and the ability to sequence multiple samples per run.Turnaround time must be no more than 10 15 business days from receipt of libraries.3. Cost Efficiency with Fixed...
Documents :