- Country: USA
- Summary: Alligator Snapping Turtle Edna Analysis from Filtered Water Samples
- UST Ref No: 104844137
- Deadline: 06 Aug 2024
- Financier: Self Financed
- Purchaser Ownership: Government
- Tender Value: Refer Document
- Notice Type: Tender
- Document Ref. No.: 1132837
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- Description:
- Description
The proposed work is to analyze samples for a single species, the Alligator Snapping Turtle, for environmental DNA (eDNA). Water samples (135 total) were collected from lakes and streams and filtered in the laboratory using analytical filter funnels containing 0.45um filters. Filters, containing eDNA, were removed from filter funnels and placed in labeled microcentrifuge tubes and the tubes were placed in a deep freeze. Each microcentrifuge tube contains 2 filters from the same water sample. Samples will be shipped in an insulated container, containing cold packs, to a laboratory for analysis. Each tube should be considered as an independent sample.
Laboratory is required to detect sequences of Alligator Snapping Turtle DNA from filter medium and ensure no cross contamination of samples. Laboratory must provide a detailed report showing CT scores for each run (3 runs per sample) and if each sample has a positive or negative result for Alligator Snapping Turtle DNA. All sample results must remain distinct for future statistical analysis.
The contractor must extract water filters using the Zymo Quick Miniprep Kit extraction kits (Zymo Research, Orange California) following a modification of the Spear et al. (2015) protocol. Next, they must amplify the DNA in a qPCR reaction using primers and thermocycler profile modified from Feist et al. (2018) to work with samples throughout the range of Alligator Snapping Turtles. The contractor must visualize amplification using a QuantStudio3 machine (Thermo Fisher Scientific, Waltham MA). The samples must be run with a negative water control and internal positive control designed to capture the genetic variation present across the range of Alligator Snapping Turtles (interspecific and intraspecific)
Vendors/laboratories must submit detailed capability statements that demonstrate how the same testing methods described above could be used. Capability statements must also show how results would ensure co... - Documents:
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CLICK HERE TO LOGINAlligator Snapping Turtle Edna Analysis from Filtered Water Samples - USA Tender
The AGRICULTURE, DEPARTMENT OF, a Government sector organization in USA, has announced a new tender for Alligator Snapping Turtle Edna Analysis from Filtered Water Samples. This tender is published on USARFP under UST Ref No: 104844137 and is categorized as a Tender. Interested and eligible suppliers are invited to participate by reviewing the tender documents and submitting their bids before the deadline on 2024-08-06.
The estimated tender value is Refer Document, and full details, including technical specifications and submission requirements, are provided in the official tender documents. Ensure all submissions meet the criteria outlined to be considered for evaluation.
