- Country: USA
- Summary: Intent to Sole Source Dna Sequencing and Fluidigm Access Array
- UST Ref No: 104154467
- Deadline: 18 Jul 2024
- Financier: Self Financed
- Purchaser Ownership: Government
- Tender Value: Refer Document
- Notice Type: Tender
- Document Ref. No.:
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- Description:
- Description
This is a Notice of Intent to sole source. Requests for a solicitation will receive no response. This proposed contract action is published to notify potential sources that the National Forest Service, Pacific Northwest Research Station intends to award a sole source firm-fixed price contract, on behalf of the USDA Forest Service. For the work desired they will be assessing environmental DNA (eDNA) samples using Fluidigm microfluidic amplification and eDNA metabarcoding. USFS PNW Research Station workers will provide extracted eDNA samples and specially designed primers to the vendor. The vendor will then amplify the targets on their Fluidigm instrument (a very specialized piece of machinery) and then sequence the amplified DNA on their NovaSeq 6000 system. The deliverables on the contract are a datafiles containing millions of demultiplexed amplified sequences.
The Corvallis Genomics Team have spent years developing an environmental monitoring assay. The basic process analyzes DNA from environmental samples and runs them through a proprietary protocol on the Fluidigm Access Array™ instrument. This instrument performs 2,304 simultaneous enzymatic reactions, labels the products of the reactions, and then elutes the products for DNA sequencing on a next-generation DNA sequencer. The team at the Roy J. Carver Center has extensive experience conducting Fluidigm experiments. To avoid repeating preliminary work (which would accrue additional costs and labor) to develop specialized protocol at a different facility it is the desire of the Genomics Team to continue to work with the University of Illinois Roy J. Carver Center.This work included hundreds of samples submitted as study batches. Each study set is equivalent to
10-13 plates of 48 samples amplified on the Access Array at a time followed by sequencing on the NovaSeq for subsequent bioinformatic analysis. The current request will process two more data sets using the same technology only in la... - Documents:
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CLICK HERE TO LOGINIntent to Sole Source Dna Sequencing and Fluidigm Access Array - USA Tender
The AGRICULTURE, DEPARTMENT OF, a Government sector organization in USA, has announced a new tender for Intent to Sole Source Dna Sequencing and Fluidigm Access Array. This tender is published on USARFP under UST Ref No: 104154467 and is categorized as a Tender. Interested and eligible suppliers are invited to participate by reviewing the tender documents and submitting their bids before the deadline on 2024-07-18.
The estimated tender value is Refer Document, and full details, including technical specifications and submission requirements, are provided in the official tender documents. Ensure all submissions meet the criteria outlined to be considered for evaluation.
