- Country: USA
- Summary: Modification - Q & A for Advanced Genetic Sequencing
- UST Ref No: 120758888
- Deadline: 16 Jun 2025
- Financier: Self Financed
- Purchaser Ownership: Government
- Tender Value: Refer Document
- Notice Type: Tender
- Document Ref. No.: 36C24E25Q0045
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- Description:
- Description
RFQ 36C24E25Q0045Advanced Genetic Sequencing Solicitation Clarification and Question & Answer**Quote submission is extended from Wednesday, June 11, 2025, to no later than 11:59 EST, Monday, June 16, 2025.**Quotes shall be submitted via email to millicent.covert@va.gov. Offerors must reference Solicitation 36C24E25Q0045 in the subject line of the email. No telephone requests for information will be considered. Incomplete packages will be considered nonresponsive._______________________________________________________________________1. For the "spatially segregated mouse brain and lung" samples, please clarify their processing prior to shipment. Specifically, will they be fresh-frozen (snap-frozen) or fixed-frozen (e.g., briefly fixed and then frozen)? The spatially segregated phrase refers to dissection of brain and lung regions prior to dissociation. No spatial transcriptomic techniques will be performed.Tissues are enzymatically dissociated into single-cell suspensions, then fixed and frozen in cryovials using Parse Biosciences methanol-based fixation protocol.2. If able, can you also comment on: a) Tissue Details: Expected section thickness, mounting method, and clarity on section orientation (e.g., anatomical landmarks, images). Not applicable, no sectioning or mounting will be involved. Only single-cell suspensions will be used.b) Quality & Shipping: Anticipated RNA Integrity Number (RIN), detailed shipping conditions (e.g., dry ice amount, packaging), and frequency of shipments. Cells are fixed at our facility prior to shipment using Parse Evercode protocols.Shipping: Frozen in cryovials on dry ice using validated shippers.Shipments are expected 2 3 times per year, based on project progress.c) Histology: Will accompanying H&E stained slides or images be provided for morphological context? N/AMorphological context (e.g., H&E slides or images) may be provided separately for selected samples, but is not required for scRNA-seq processing.3. For the... - Documents:
Attachment-1-Statement-of-Work_updated.docx
RFQ-36C24E25Q0045-0001-SF-30_Modification.docx
Attachment-2-Price-Cost-Schedule.docx
Attachment-1-Statement-of-Work.docx
SF30-Solicitation-36C24E25Q0045.docx
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CLICK HERE TO LOGINModification - Q & A for Advanced Genetic Sequencing - USA Tender
The VETERANS AFFAIRS, DEPARTMENT OF, a Government sector organization in USA, has announced a new tender for Modification - Q & A for Advanced Genetic Sequencing. This tender is published on USARFP under UST Ref No: 120758888 and is categorized as a Tender. Interested and eligible suppliers are invited to participate by reviewing the tender documents and submitting their bids before the deadline on 2025-06-16.
The estimated tender value is Refer Document, and full details, including technical specifications and submission requirements, are provided in the official tender documents. Ensure all submissions meet the criteria outlined to be considered for evaluation.
