- Country: USA
- Summary: Modification - Q & A for Advanced Genetic Sequencing
- UST Ref No: 121521700
- Deadline: 26 Jun 2025
- Financier: Self Financed
- Purchaser Ownership: Government
- Tender Value: Refer Document
- Notice Type: Tender
- Document Ref. No.: 36C24E25Q0045
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- Description:
- Description
RFQ 36C24E25Q0045Advanced Genetic Sequencing Solicitation Clarification and Question & Answer, PART 2
**Quote submission is extended to no later than 11:59AM EST, Thursday, June 26, 2025.**
Quotes shall be submitted via email to millicent.covert@va.gov. Offerors must reference Solicitation 36C24E25Q0045 in the subject line of the email. No telephone requests for information will be considered. Incomplete packages will be considered nonresponsive.
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Response to Question 1: Scope, Library Prep, and Sequencing Capacity
We appreciate your request for clarification and have refined the expectations to ensure alignment with the full Statement of Work (SOW). The following applies:1. Workflow Scope - Full Single-Cell RNA-seq Services Required
The requirement includes complete execution of the Parse Biosciences single-cell RNA-seq pipeline, starting from fixed single-cell suspensions. This encompasses:- Sample intake and QC- Cell barcoding and library preparation using Parse WT or MEGA kits- Library quality control- High-throughput sequencing on NovaSeq X Plus PE150 (25B lanes)- Full bioinformatics pipeline (alignment, clustering, DEG, pathway analysis)Pre-made libraries are not in scope, and the contractor must have demonstrated experience handling Parse Biosciences workflows end-to-end, including immediate sequencing upon library completion without outsourcing.2. Library QC and Kit Considerations
Library QC is embedded within the full workflow and must be included in the price per library. The contractor should have:- Infrastructure and staffing to process up to 20 libraries/year in multiple independent batches (2-6 per shipment)- Access to and experience with Parse WT and MEGA kits, with the ability to scale per batch and adjust based on submission schedule without delays or combining batches3. Sequencing Specifications and Lane Requirements
Sequencing must reach 30,0... - Documents:
Attachment-1-Statement-of-Work_updated.docx
RFQ-36C24E25Q0045-0001-SF-30_Modification.docx
Attachment-2-Price-Cost-Schedule.docx
Attachment-1-Statement-of-Work.docx
SF30-Solicitation-36C24E25Q0045.docx
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CLICK HERE TO LOGINModification - Q & A for Advanced Genetic Sequencing - USA Tender
The VETERANS AFFAIRS, DEPARTMENT OF, a Government sector organization in USA, has announced a new tender for Modification - Q & A for Advanced Genetic Sequencing. This tender is published on USARFP under UST Ref No: 121521700 and is categorized as a Tender. Interested and eligible suppliers are invited to participate by reviewing the tender documents and submitting their bids before the deadline on 2025-06-26.
The estimated tender value is Refer Document, and full details, including technical specifications and submission requirements, are provided in the official tender documents. Ensure all submissions meet the criteria outlined to be considered for evaluation.
