Tender Details

Country: USA
Summary: PCR Amplify DNA Extracts and Sequencing
UST Ref No: 125284132
Deadline: 11 Sep 2025
Financier: Self Financed
Purchaser Ownership: Government
Tender Value: Refer Document
Notice Type: Tender
Document Ref. No.: 143021-25-0187
Purchaser's Detail:
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Description:
Description
The U.S. Department of Commerce (DOC), National Oceanic and Atmospheric Administration (NOAA), Western Acquisition Division (WAD) intends to award, on a sole source basis, a contract to the Cold Spring Harbor Laboratory with a 10-month period of performance under the authority of FAR 13.106-1(b)(1).
The Northeast Fisheries Science Center (NEFSC) leads efforts to use contemporary molecular techniques in fisheries and aquaculture research projects. Previously, a NERAP/EBFM-funded proposal titled “Trophic ecology of longfin inshore squid (Dorytheuthis pealeii) and northern shortfin squid (Illex illecebrosus) in the NW Atlantic with enhanced fish prey identification” included the PCR amplification and sequencing of 300 DNA extracts by Cold Spring Harbor Laboratory (Cold Spring Harbor, NY; Purchase Order # 1333MF23PNFFM0137). This contract was successfully completed in September 2024.
The NEFSC leads efforts to use contemporary molecular techniques in fisheries and aquaculture research projects. Critical DNA sequencing is needed for a federally supported research project on squid ecology to move forward, as well as advances in molecular tools for managing squid and related fisheries
The NEFSC has a requirement for the processing of samples at NEFSC using a lab technique used to make many copies of a specific piece of DNA to examine the ecology of longfin and shortfin squid. This work builds upon an existing project, which will provide data used for fishery management of the valuable and emerging squid fishery.
Given the sensitivity of the approach to identify prey with molecular techniques, a recurring presence of suspected contamination was seen in the resulting data although the direct source of this contamination could not be fully identified. As a result of this, the Government needs a second round of sequencing for another 300 DNA extracts to apply the same methods and procedures previously used. This will help identify the direct so...
Documents:

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PCR Amplify DNA Extracts and Sequencing - USA Tender

The COMMERCE, DEPARTMENT OF | NATIONAL OCEANIC AND ATMOSPHERIC ADMINISTRATION, a Government sector organization in USA, has announced a new tender for PCR Amplify DNA Extracts and Sequencing. This tender is published on USARFP under UST Ref No: 125284132 and is categorized as a Tender. Interested and eligible suppliers are invited to participate by reviewing the tender documents and submitting their bids before the deadline on 2025-09-11.

The estimated tender value is Refer Document, and full details, including technical specifications and submission requirements, are provided in the official tender documents. Ensure all submissions meet the criteria outlined to be considered for evaluation.