- Country: USA
- Summary: Services for Probe Design and Rnascope Brand Reagent Kits
- UST Ref No: 81724167
- Deadline: 22 Apr 2023
- Financier: Self Financed
- Purchaser Ownership: Government
- Tender Value: Refer Document
- Notice Type: Tender
- Document Ref. No.: NOI-NIAID-2169561
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- Description:
- Description
This is a Notice of Intent, not a request for proposal. The National Institute of Allergy and Infectious Diseases (NIAID) of the National Institutes of Health (NIH) intends to negotiate on an other than full and open competition basis with a Advanced Cell Diagnostics, Newark, CA
These services and supplies are required for RNA in situ hybridization.Qty: 23 Bioinformatics service fee for probe designs (Multiple) Qty: 23 RNASCOPE brand probes (Multiple)Qty: 1 RNASCOPE brand Multiplex reagent kit #323270
Sole Source Justification: Advanced Cell Diagnostics, Inc. (ACD) is the sole supplier of RNAscope® probes and reagent Kits.
This projects demands RNA in situ hybridization to validate 23 target markers and visualize the individual cellular clusters identified in the single-cell RNA-seq analysis. RNAscope enables as much as 400-fold improvement over conventional RNA in situ hybridization, resulting in high detection sensitivity, thus RNAscope technology is the best choice to accomplish our goals for this project. RNAscope was designed to amplify target-specific signal without also amplifying the background signal, resulting in marked improvement in signal-to-noise ratio. This is accomplished by manufacture`s patented double Z-probe design for in situ hybridization, where two independent probes (a double Z probe pair) are required to hybridize in tandem to the target sequence for signal amplification to occur. Since it is highly unlikely that two independent probes will hybridize to a nonspecific target right next to each other, this design concept ensures highly selective amplification of target-specific signals, therefore improving both sensitivity and specificity. A Preamplifier (PreAmp) molecule hybridizes to each double Z probe pair, and then multiple Amplifier (AMP) molecules hybridize to each Preamp. Finally, multiple HRP-labeled Label Probes hybridized to each AMP. DAB substrate is added for colorimetric detection of target RNA. Fluor... - Documents:
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CLICK HERE TO LOGINServices for Probe Design and Rnascope Brand Reagent Kits - USA Tender
The HEALTH AND HUMAN SERVICES, DEPARTMENT OF, a Government sector organization in USA, has announced a new tender for Services for Probe Design and Rnascope Brand Reagent Kits. This tender is published on USARFP under UST Ref No: 81724167 and is categorized as a Tender. Interested and eligible suppliers are invited to participate by reviewing the tender documents and submitting their bids before the deadline on 2023-04-22.
The estimated tender value is Refer Document, and full details, including technical specifications and submission requirements, are provided in the official tender documents. Ensure all submissions meet the criteria outlined to be considered for evaluation.
