- Country: USA
- Summary: Sources Sought for Sequencing of Wild Pea, Pisum Fulvum - Pfna27 Reference Genome
- UST Ref No: 102679561
- Deadline: 19 Jun 2024
- Financier: Self Financed
- Purchaser Ownership: Government
- Tender Value: Refer Document
- Notice Type: Tender
- Document Ref. No.: 12905B24SS00040
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- Description:
- Description
This is a Sources Sought notice Only. This is NOT a solicitation for proposals, proposal abstracts, or quotations. This Sources Sought is issued for information and planning purposes only as part of USDA ARS market research and does not itself constitute an RFQ. This requirement is covered under NAICS code 541714. The purpose of this notice is to obtain information regarding the availability and capability of qualified sources; Small Businesses; HUBZone small business; service-disabled, veteran-owned small business; 8(a) small business; veteran-owned small business; woman-owned small business; or small disadvantaged businesses; Responses to the information requested will assist the Government in determining the appropriate acquisition method, including whether a set-aside is possible.
The Sequence and assembly of high-quality complete reference genome with annotated transcriptome of USDA ARS Accession Number PI 343996 pea Pisum sativum subspecies elatius using the PacBio Revio system high-density 25 million ZMW SMRT cells delivering HiFi reads. Work to include library construction frozen plant tissue using the Dovetail Hi-C Kit. Expected product will include the yield for CCS sequencing of 60-90Gb for the pea DNA per lane and a final yield of 2.5 billion read pairs and 750Gb of raw data per lane. Gene and protein annotation will be included. In addition, RNA prep from frozen plant tissue (8 different plant tissues, see list below), RNASeq library construction and sequencing of up to 40 million read pairs per sample using Stranded HT, Illumina Sequencing on a NovaSeq 6000 PE150. Data for the complete genome annotation will be delivered as GFF, cDNA and protein files for each pea sample (haplotype). Base pair call accuracy of 99.9% (Q30 or greater).
For each genotype, the sequencing costs $189.00 for first 10 Gb and $95.00 for each incremental 10Gb. Therefore, for 40Gb data, the total cost per genotype is $474 [189 + (3*95)]. Total c... - Documents:
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CLICK HERE TO LOGINSources Sought for Sequencing of Wild Pea, Pisum Fulvum - Pfna27 Reference Genome - USA Tender
The AGRICULTURE, DEPARTMENT OF, a Government sector organization in USA, has announced a new tender for Sources Sought for Sequencing of Wild Pea, Pisum Fulvum - Pfna27 Reference Genome. This tender is published on USARFP under UST Ref No: 102679561 and is categorized as a Tender. Interested and eligible suppliers are invited to participate by reviewing the tender documents and submitting their bids before the deadline on 2024-06-19.
The estimated tender value is Refer Document, and full details, including technical specifications and submission requirements, are provided in the official tender documents. Ensure all submissions meet the criteria outlined to be considered for evaluation.
